The terms spheroids and organoids are not new and they are often used interchangeably due to their similarities. They both refer to cells cultured in 3 dimensions (3D), but there are key differences between the two. These have important implications for their application.
A spheroid is a simple 3D cell culture often generated from a single cell type, or cell aggregates, freely floating typically in ultra-low attachment (ULA) plates. An organoid is a 3D culture of tissue/organ-specific cell types, usually originating from stem cells and progenitors embedded within an ECM hydrogel matrix.
Organoids are highly complex and better mimic the in vivo environment when compared to spheroids or traditional 2D culture systems. Consequently, organoids are gaining increasing interest and are used widely to model diseased tissues, and for testing the response, toxicity and efficacy of new drug entities.
Excitingly, we have shown recently that our animal free PeptiGels® provide a suitable platform to support the growth of a number of different spheroids and organoids, including gut, liver, kidney, cardiac and cancer. Moreover, they give reliable and reproducible data for in vitro 3D models of human development and disease, hence are finding application within drug discovery.
Are you working in this area and interested in increasing your data turn-around time in a cost effective way and gain reproducible and reliable results? If yes, then get in touch now as our expert team is here and happy to provide you with key insights and guidance.
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